Protein sumoylation assay

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August undefined, 2024

WebSep 24, 2015 · University of California, Riverside. Ph. DBioengineering. 2007 - 2012. Activities and Societies: Research in quantitative FRET … WebAug 17, 2015 · Protein SUMOylation has emerged as an important regulatory event, particularly in nuclear processes such as transcriptional control and DNA repair. ... Information regarding the plasmids, cell lines, transfections, and reporter gene assays used in this study can be found in SI Materials and Methods. Microarray Analysis, RT-PCR, …

Compartmentalization of the SUMO/RNF4 pathway by SLX4

WebFeb 2, 2016 · This unit describes two different assays for determining whether a particular protein is sumoylated: the first method employs immunoprecipitation of the protein … WebGlobal Protein Sumoylation Assay Kit (ab115131) Specific References (2) Description: Global Protein Sumoylation Assay Kit. Reactivity: Mouse, Rat, Human (predicted: Mammals) Sample type: Cell culture extracts, Tissue Extracts. HDAC1 Sumoylation Assay Kit (ab115132) Description: HDAC1 Sumoylation Assay Kit. Reactivity: Mouse, Rat, … thl37s2 仕様

Frontiers SUMOylation Is Essential for Dengue Virus Replication …

WebSumoylation of the targeted proteins are detected by recognition of SUMO conjugated to these proteins with an anti-SUMO antibody. The ratio or intensity of the sumoylation, which is proportional to the conjugated SUMO amount, can be quantified through the signal report-color development system. 2. ASSAY SUMMARY Nuclear Extract WebApr 27, 2024 · Protein SUMOylation regulates a wide variety of cellular processes and is important for controlling virus replication. Earlier studies suggest that dengue virus envelope protein interacts with Ubc9, the sole E2-conjugating enzyme required for protein SUMOylation in mammalian cells. Web2 days ago · Protein SUMOylation plays important roles in the DDR, ... the supernatant was collected and the amount of protein was quantified using the Quick Start Bradford protein assay kit. Laemmli buffer was added, and proteins were boiled 5 min at 95 °C. 40μg of protein samples were resolved on precast SDS-PAGE gels (4-15% and 10%) and … thl37s2

SUMO enables substrate selectivity by mitogen-activated protein ... - PNAS

Detection of sumoylated proteins - PubMed

WebMar 23, 2024 · SUMOylation is a type of posttranslational modification whereby the small ubiquitin-like modifier (SUMO) is transferred onto target proteins ( Verma et al., 2024 ). Although the structures of ubiquitin and SUMO are similar, they may play antagonistic functions in the regulation of their targets ( Ulrich, 2005 ). WebJan 13, 2024 · SUMOylation inhibitors rescue FXR signaling and thereby increasing the efficacy of OCA against HSC activation and fibrosis. FXR upregulates Perilipin-1, a direct target gene of FXR, to stabilize lipid droplets and thereby prevent HSC activation. th-l42d2WebNov 10, 2024 · Nonstructural protein 1 (NS1) of the influenza A virus is a major contributor to the virulence of the seasonal influenza A viruses, in part because it interferes with host viral defense mechanisms. ... In this study, we developed a novel FRET assay to assess SUMOylation. Using this assay, we demonstrated that the lysine residue K131 in the ... thl42d2

"WebApr 27, 2024 · Quantitative FRET assays are capable of determining various biochemical parameters (e.g., the protein interaction dissociation constant, enzymatic velocity, and K m) of the SUMOylation machinery ... " - Protein sumoylation assay

Protein sumoylation assay

Yan LIU - Director of Research - Nova Therapeutics

WebTo perform an in vitro SUMOylation assay, begin with a tube containing a mixture of the substrate protein - p53, enzymes, and SUMO peptides, and incubate to induce SUMOylation. Supplement the tube with sodium dodecyl sulfate - SDS-containing buffer, terminating the reaction. WebVivo Universal Protein Sumoylation Assay Kit addresses this problem, using a proprietary and unique procedure to measure in vivo protein sumoylation. The kit has the following features: • Fast procedure, which can be finished within 5 hours. • One-step colorimetric assay without the need for affinity chromatography and Western blotting.

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WebJan 24, 2024 · 7. Assay Protocol A. SUMOylation Assay Assay described for the SUMOylation of purified target proteins and control RG1 protein in vitro. Note: Assay conditions tested/optimized for a range of target proteins, including p53 tumor repressor and interferon inducible protein SP100, but may require adjustment for alternative enzymes. WebMar 3, 2024 · SUMO Proteomics Analyses Identify Protein Inhibitor of Activated STAT-Mediated Regulatory Networks Involved in Cell Cycle and Cell Proliferation - PMC Back to Top Skip to main content An official website of the United States government Here's how you know The .gov means it’s official. Federal government websites often end in .gov or …

WebSUMOylation is a critical regulator of a broad range of cellular processes, and is thought to do so in part by modulation of protein interaction. To comprehensively identify human proteins whose interaction is modulated by SUMOylation, we developed an in vitro binding assay using human proteome microarrays to identify targets of SUMO1 and SUMO2. Websumoylation of the I B protein (Fig. 3A Left), the IE1 proteinfrom cytomegalovirus (Center), or the Sp100 protein (Right). Bycontrast, SUMO modification of the c-Jun transcription factorwas induced by both PIASx and PIAS1. As shown in Fig. 3B,under conditions of low Ubc9 concentration, no basal sumoy-lation of c-Jun was observed in this assay. H...

WebJan 24, 2024 · SUMOylation Assay Assay described for the SUMOylation of purified target proteins and control RG1 protein in vitro. Note: Assay conditions tested/optimized for a range of target proteins, including p53 tumor repressor and interferon inducible protein SP100, but may require adjustment for alternative enzymes. WebJan 15, 2014 · 2. PTP1B sumoylation. We identified PIAS1, SUMO E3 ligase as PTP1B interacting protein by a yeast two-hybrid assay [8].This interaction was confirmed by co-immunoprecipitation studies, using wild-type PTP1B mouse embryonic fibroblasts (MEFs) or Ptp1b-null MEFs.We then showed that PTP1B is in fact sumoylated in cells, mainly at the …

WebProtein SUMOylation regulates the activity of a wide range of cellular substrates, and the identification of small ubiquitin-related modifier (SUMO)-modified sites is often required to …

WebJun 11, 2010 · Protein sumoylation is mediated by activating (E1), conjugating (E2), and ligating (E3) enzymes . Ubc9 is the only identified SUMO E2 conjugating enzyme. However, several sequence-specific SUMO E3 ligases have been identified. ... The in vitro sumoylation assay was carried out using an in vitro sumoylation kit (LAE Biotech … thl37s2 取説WebApr 22, 2013 · SUMO (Small Ubiquitin MOdifier) is an ubiquitin-like protein involved in post- translational modification of critical cellular factors controlling protein localisation, … thl4002s tecnoliteWebBelow is the general protocol for expressing the recombinant proteins needed for the in vitro sumoylation assay. Each recombinant protein to be used in this assay needs to be … th-l42e5 仕様WebThe localizations of aggrecan and Col-II, as well as the SUMOylation modification of these proteins were analyzed using immunofluorescence and immunoprecipitation assays, respectively.Results: Our results showed that a proinflammatory cytokine interleukin-1β induced the OA model and desumoylation of aggrecan and collagen type II because the ... thl 4WebApr 22, 2013 · SUMO (Small Ubiquitin MOdifier) is an ubiquitin-like protein involved in post- translational modification of critical cellular factors controlling protein localisation, transcription, DNA repair or cell cycle progression among other functions 1,2.Protein SUMOylation is defined as the covalent modification of target proteins with SUMO … thl42e6z reviewsWebJan 28, 2016 · Protein SUMOylation regulates the activity of a wide range of cellular substrates, and the identification of small ubiquitin-related modifier (SUMO)-modified … th-l42e60 故障 th-l42e60 発売日